masculinization of blue hap (sciaenochromis ahli) treated with 17α-methyltestosterone

the objective of this study was to determine the efficacy of two procedures i.e. feeding and immersion followed by feeding, for masculinizing blue hap. two experiments (experiments i and ii) were conducted. in experiment i, feeding hormonal treatment was applied on post-yolk sac stage fry (10 days after fertilization at 27ºc). dosages of 30mg (group a) and 60mg (group b) of 17α-methyltestosterone per kg of food were used during 60-day periods (40 fry per treatment). the control group i.e. group c, received no hormone. in experiment ii, one day post hatching larvae were exposed to an immersion treatment in 17α-methyltestosterone at 1000 μg/l up to 2 h (16 larvae per treatment). later, at the first day of post-yolk sac stage, the treatment was followed by oral procedure which was divided in 2 groups: group d and group e. in group d, 30 mg 17α-methyltestosterone per kg of food was used during a 60-day period. in group e, fry received 60 mg of hormone per kg of food during the same period. the control group i.e. group f, did not receive any hormone. change in sex proportion within each experiment as well as between experiments was analyzed by chi-square test (p